I am an evolutionary biologist interested in molecular mechanisms and population dynamics underlying the adaptation of bacterial communities to heterogeneous environments. Find out more about some of my projects at ORCiD Researcher Profile.
I have worked with both Pseudomonas fluorescens and E. coli and used the two model systems to study adaptation of bacterial species in fluctuating environments. I have used the two model systems to investigate biochemical and metabolic pathways.
I have used genetic engineering techniques such as strand overlap extension PCR (SOE-PCR; scar-free) and Lambda Red Recombineering for generating mutants of P. fluorescens and E. coli, respectively. I have also optimised the scar-free technique for non-model strains of P. fluorescens.
I have done whole genome sequencing in P. fluorescens and RNA sequencing and ChIP sequencing in E. coli. I have developed analysis pipelines for sequencing data using bash and R. I have also done qRT-PCR for expression studies in P. fluorescens.
During my Masters, I isolated a polyvalent phage and characterized structural (TEM), molecular and burst kinetic properties.
I investigated the physiological implication of changing codon usage patterns and characterized biofilm formation at the air-liquid interface in P. fluorescens.
In my current project, I am investigating the adaptation of bacteria to fluctuating conditions. I am also developing analysis pipelines using R and bash for RNAseq and ChIPseq data.
